In SDN-3 applications of CRISPR/Cas9 in mice, it was shown that DNA templates are incorporated multiple times within target sequences and cannot be detected using simple standard analysis methods (PCR, polymerase chain reaction). In the study, the authors intended to create mice containing a conditional knockout of a certain gene. For this purpose, together with […]
This paper examines where exactly CRISPR/Cas constructs are integrated into the genome of soy plants and how these, together with the intended changes resulting from their mode of action, are passed on to the following generation(s). Interestingly, it is not only about whether and how the CRISPR gene scissors cut in the genome, but also […]
Scientist at the U.S. Food and Drug Administration (FDA) evaluated whole genome sequencing data of cattle that have been genetically modified so that they do not grow horns (polled). The cattle were originally modified with TALENs and the polled property was introduced into the cattle genome using a SDN-2/3 approach (original publication: Carlson, DF et […]
In this study the bacterium Snodgrassella alvi, known as a symbiotic bacterium in the gut of honey bees, was genetically modified to produce specific dsRNAs to induce RNA interference (RNAi) in varroa mites and the deformed wing virus (DWV). Apparently honey bees that contain genetically modified S.alvi are protected from varroa and DWV. RNAi is […]
This publication describes tomatoes and physalis plants from the Solanaceae family that have been modified by CRISPR/Cas so that they are much smaller in size and flower earlier than other tomatoes. 3 genes were knocked out in the tomato using CRISPR/Cas, which are necessary for the time of flowering, the length of the stem and […]
Scientists detected structural irregularities, unwanted changes and T-DNA (transfer DNA from plasmids) integration by using whole genome sequencing methods after transformation of the plant using an Agrobacterium tumefaciens plasmid. They used a novel sequencing method called Nanopore long-read DNA sequencing technology, that enables the sequencing of long stretches of DNA. Apparently, the integration of T-DNA […]
This study systematically shows, in various human cell lines, that cytosine base editors (CBEs), which are supposed to enzimatically convert cytosines to thymines in specific target sites of the genome, also cause many unintended changes at the level of the RNA. Base editors are based on a dead Cas9 enzyme that can still recognize the […]
Proteins are composed of 20 amino acids. Those amino acids are determined by a total of 64 codons. Three base pairs of the DNA, also named a codon, are encoding one amino acid. Eighteen amino acids contain more than one codon, which can have an impact on gene-expression. In this study, an Escherichia coli strain […]
The paper examines risks posed by plants produced using new genetic modification techniques (nGMs). For this purpose, a literature survey was conducted from January 2011 to June 2017 and important aspects for a suitable risk assessment were discussed using market-orientated applications in plants. The authors conclude that even small changes in the genome and the […]
Prime editing, a new variant of the CRISPR/Cas toolbox, enables the introduction of deletions, insertions and all 12 options to convert one base enzymatically into another at target sequence(s) in the genome of target organisms. In prime editing, a single strand of the DNA is cut to reduce the occurrence of off-target effects. In addition, […]
