Pervasive head-to-tail insertions of DNA templates mask desired CRISPR-Cas9-mediated genome editing events
In SDN-3 applications of CRISPR/Cas9 in mice, it was shown that DNA templates are incorporated multiple times within target sequences and cannot be detected using simple standard analysis methods (PCR, polymerase chain reaction). In the study, the authors intended to create mice containing a conditional knockout of a certain gene. For this purpose, together with […]