Horizon Scanning

During the horizon scanning process, scientific publications are screened and evaluated to identify and analyze possible environmental effects of new technical developments and their applications in the field of genetic engineering/biotechnology considering the precautionary principle. The results of the horizon scanning are made available to the public in form of short summaries. It is an ongoing collection of current literature that does not claim to be complete and is continuously updated.
The current focus of the horizon scanning process can be found → here and a glossary with explanations of the most important terms can be found  here

A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae

In this study, a gene drive in malaria-transmitting mosquitoes (Anopheles gambiae) is presented in which an already described CRISPR/Cas-based gene drive (original publication: Kyrou K, Hammond AM, Galizi R, Kranjc N, Burt A, Beaghton AK, Nolan T, Crisanti A (2018) A CRISPR-Cas9 gene drive targeting doublesex causes complete population suppression in caged A. gambiae mosquitoes. […]

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Metabolomics should be deployed in the identification and characterization of gene-edited crops

Methods such as nuclear magnetic resonance (NMR) and gas or liquid mass spectrometry allow the detection of changes in the metabolism (e.g. production of chemical compounds) of plant cells. The collective term of these methods is „metabolomics“. Metabolomics methods can also be grouped together with genomics (analysis methods to examine the entire DNA sequence of […]

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Pervasive head-to-tail insertions of DNA templates mask desired CRISPR-Cas9-mediated genome editing events

In SDN-3 applications of CRISPR/Cas9 in mice, it was shown that DNA templates are incorporated multiple times within target sequences and cannot be detected using simple standard analysis methods (PCR, polymerase chain reaction). In the study, the authors intended to create mice containing a conditional knockout of a certain gene. For this purpose, together with […]

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Template plasmid integration in germline genome-edited cattle

Scientist at the U.S. Food and Drug Administration (FDA) evaluated whole genome sequencing data of cattle that have been genetically modified so that they do not grow horns (polled). The cattle were originally modified with TALENs and the polled property was introduced into the cattle genome using a SDN-2/3 approach (original publication: Carlson, DF et […]

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The complex architecture and epigenomic impact of plant T-DNA insertions

Scientists detected structural irregularities, unwanted changes and T-DNA (transfer DNA from plasmids) integration by using whole genome sequencing methods after transformation of the plant using an Agrobacterium tumefaciens plasmid. They used a novel sequencing method called Nanopore long-read DNA sequencing technology, that enables the sequencing of long stretches of DNA. Apparently, the integration of T-DNA […]

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