On-target effects are unintended changes in the genome of genome-edited target organisms that can occur when applying the gene scissors CRISPR/Cas. On-target effects include major rearrangements of the genome, such as restructuring large parts of the DNA, large insertions of DNA sequences or the deletion of DNA sections. So far it has not been fully […]
In the present study, maize plants are generated containing the Cas9 gene scissors and guide RNAs for target regions in two different genes in wheat. The target genes BRI1 and SD1 regulate the growth of wheat. The scientists use 5 transgenic maize plants for each target region in the wheat (targets determined by the guide […]
Major unintended restructuring of the genome at or in the vicinity of the target sequence are known as so-called „on-target effects“. Major restructuring in the genome can be, for example, large deletions, that is, very large pieces of DNA are deleted or large DNA sequences are unintentionally inserted at the target sequence. In previous studies, […]
So far it has not yet been possible to clearly demonstrate whether a new change in the genome of plants has arisen through an application of genome editing or whether a naturally occurring mutation is present. This study by Biswas et al. 2020 describes a method to detect both CRISPR/Cas-induced and naturally occurring mutations. Changes […]
A trait that is often changed in rice so resulting rice plants have a smaller size and a higher yield is caused by changes in the so-called semi-dwarf 1 (SD1) gene. This gene is modified in various rice elite lines in the present study with CRISPR/Cas9 and examined in detail in subsequent generations for unintended […]
With the use of CRISPR/Cas9, scientists have succeeded in exchanging larger sections of DNA between two different chromosomes in the model plant Arabidopsis thaliana. To do this, they used two gRNAs that guided the gene scissors to a target region at the first and second chromosomes of the plant. Subsequently, the gene scissors introduce a […]
In this study, a gene drive in malaria-transmitting mosquitoes (Anopheles gambiae) is presented in which an already described CRISPR/Cas-based gene drive (original publication: Kyrou K, Hammond AM, Galizi R, Kranjc N, Burt A, Beaghton AK, Nolan T, Crisanti A (2018) A CRISPR-Cas9 gene drive targeting doublesex causes complete population suppression in caged A. gambiae mosquitoes. […]
Methods such as nuclear magnetic resonance (NMR) and gas or liquid mass spectrometry allow the detection of changes in the metabolism (e.g. production of chemical compounds) of plant cells. The collective term of these methods is „metabolomics“. Metabolomics methods can also be grouped together with genomics (analysis methods to examine the entire DNA sequence of […]
In vitro cell culture is a bottleneck in the production of transgenic and genome-edited plants: in only a few species it is possible to develop new, modified plants from cell cultures. Furthermore, It is a lengthy process in which new and unintended mutations can occur. This study here describes a new method that can be […]
In SDN-3 applications of CRISPR/Cas9 in mice, it was shown that DNA templates are incorporated multiple times within target sequences and cannot be detected using simple standard analysis methods (PCR, polymerase chain reaction). In the study, the authors intended to create mice containing a conditional knockout of a certain gene. For this purpose, together with […]
