Publications

Kawall, K., Cotter, J., Then, C. (2020) → Broadening the GMO risk assessment in the EU for genome editing technologies in agriculture. Environ Sci Eur 32, 106. doi: 10.1186/s12302-020-00361-2
The study describes the risks associated with the use of new genetic engineering methods in plants and animals. On the one hand, these are unintended changes in the genome triggered by the genetic engineering process and, on the other hand, intended interventions resulting from genome editing that are also associated with risks.
The study gives an overview of genome editing techniques and describes unintended effects that are specific to their application. Such unintended effects can be so-called “off-target effects”, i.e. unwanted changes in other parts of the genome than were actually intended. In addition, “on-target effects” can occur, i.e. where unintentional changes occur in or near the target region of the genome. This includes, for example, the unintentional incorporation of additional DNA sequences or unintended rearrangements of the genome. In many cases, the methods of old and new genetic engineering are still combined to introduce the gene scissors into the cells. This is why unwanted effects arising from these older genetic engineering processes can also occur.
The new genome editing methods allow the genome to be changed to a degree that was previously hardly possible. Even though the changes introduced into the genome are often quite small, collectively they result in target organisms with completely new genetic combinations. It does not matter whether additional genes are introduced into the genome. The intended changes can have a significant impact on metabolic pathways and ingredients. That is why the new properties must be assessed carefully for risks even if no new genes are inserted.

 

Then, C., Kawall, K., Valenzuela, N. (2020)Spatio-temporal controllability and environmental risk assessment of genetically engineered gene drive organisms from the perspective of EU GMO Regulation Integr Environ Assess Manag. doi:10.1002/ieam.4278
Gene drive organisms pass on genetically engineered elements to their offspring more frequently than would be the case with natural rules of inheritance. The risk assessment of such gene drive organisms poses a particular challenge since subsequent generations of the genetically modified organisms can exhibit properties not observed or intended in previous generations. Such unintended effects can result from the hybridization of the gene drive construct with natural populations and/or can be triggered by changing environmental conditions. This is particularly relevant for gene drive elements which have invasive properties and typically take dozens of generations to achieve the desired effect. Under such circumstances, these so-called “next generation effects” can significantly increase spatial and temporal complexity, which is associated with a high degree of uncertainty in risk assessment. To solve these problems, we propose the introduction of a new, additional step in the risk assessment of gene drive organisms that takes the following three criteria into account: the biology of the target organisms, their naturally occurring interactions with the environment (biotic and abiotic) and the intended biological properties introduced into the organisms using genetic engineering techniques. These three criteria are merged in an additional step within risk assessment to assess the spatial-temporal controllability of gene drive organisms. The latter can be used to define so-called “cut-off” criteria in the risk analysis: if it is likely that gene drive organisms will escape spatial-temporal controllability, the risk assessment cannot be considered sufficiently reliable because it would be inconclusive. In such circumstances, the release of the gene drive organisms into the environment would not be compatible with the precautionary principle.

 

Bauer-Panskus, A., Miyazaki, J., Kawall, K., Then, C. (2020). →  Risk assessment of genetically engineered plants that can persist and propagate in the environment  Environ Sci Eur 32, 32. doi: 10.1186/s12302-020-00301-0

New challenges arise in risk assessment when genetically engineered (GE) plants can persist and propagate in the environment as well as produce viable offspring. Next generation effects can be influenced by heterogeneous genetic backgrounds and unexpected effects can be triggered in interaction with environmental conditions. Consequently, the biological characteristics of the original events cannot be regarded as sufficient to conclude on hazards that may emerge in following generations. Potential hazards identified by the European Food Safety Authority (EFSA) include exacerbating weed problems, displacement and even extinction of native plant species. However, it is concerning that EFSA only takes into account the characteristics of the original events, leaving aside issues of unexpected and unintended effects in following generations that emerge from spontaneous propagation and gene flow. From our review of the available publications and the analysis of risk assessment that was carried out, we conclude that the risk assessment of GE organisms able to persist and spontaneously propagate in the environment actually suffers from a high degree of spatio-temporal complexity causing many uncertainties. To deal with this problem, we recommend establishing ‘cut-off criteria’ in risk assessment that include factual limits of knowledge. It is proposed that these criteria are applied in a specific step within risk assessment, i.e. ‘spatio-temporal controllability’ that uses well-defined biological characteristics to delineate some of the boundaries between known and unknowns. This additional step in risk assessment will foster robustness in the process and can substantially benefit the reliability and overall conclusiveness of risk assessment and decision-making on potential releases.

 

Kawall, K. (2019).New Possibilities on the Horizon: Genome Editing Makes the Whole Genome Accessible for Changes Front. Plant Sci. 10:525. doi: 10.3389/fpls.2019.00525

The emergence of new genome editing techniques, such as site-directed nucleases, clustered regulatory interspaced short palindromic repeats (CRISPRs)/Cas9, transcription activator-like effector nucleases (TALENs) or zinc finger nucleases (ZFNs), has greatly increased the feasibility of introducing any desired changes into the genome of a target organism. The ability to target a Cas nuclease to DNA sequences with a single-guide RNA (sgRNA) has provided a dynamic tool for genome editing and is naturally derived from an adaptive immune system in bacteria and archaea. CRISPR/Cas systems are being rapidly improved and refined, thereby opening up even more possibilities. Classical plant breeding is based on genetic variations that occur naturally and is used to select plants with improved traits. Induced mutagenesis is used to enhance mutational frequency and accelerate this process. Plants have evolved cellular processes, including certain repair mechanisms that ensure DNA integrity and maintenance of distinct DNA loci. The focus of this review is on the characterization of new potentials in plant breeding through the use of CRISPR/Cas systems that eliminate natural limitations in order to induce thus far unachievable genomic changes.