Horizon Scanning

During the horizon scanning process, scientific publications are screened and evaluated to identify and analyze possible environmental effects of new technical developments and their applications in the field of genetic engineering/biotechnology considering the precautionary principle. The results of the horizon scanning are made available to the public in form of short summaries. It is an ongoing collection of current literature that does not claim to be complete and is continuously updated.
The current focus of the horizon scanning process can be found → here

Detection of Deleterious On-Target Effects after HDR-Mediated CRISPR Editing.

Major unintended restructuring of the genome at or in the vicinity of the target sequence are known as so-called „on-target effects“. Major restructuring in the genome can be, for example, large deletions, that is, very large pieces of DNA are deleted or large DNA sequences are unintentionally inserted at the target sequence. In previous studies, […]

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Effective identification of CRISPR/Cas9-induced and naturally occurred mutations in rice using a multiplex ligation-dependent probe amplification-based method.

So far it has not yet been possible to clearly demonstrate whether a new change in the genome of plants has arisen through an application of genome editing or whether a naturally occurring mutation is present. This study by Biswas et al. 2020 describes a method to detect both CRISPR/Cas-induced and naturally occurring mutations. Changes […]

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A male-biased sex-distorter gene drive for the human malaria vector Anopheles gambiae

In this study, a gene drive in malaria-transmitting mosquitoes (Anopheles gambiae) is presented in which an already described CRISPR/Cas-based gene drive (original publication: Kyrou K, Hammond AM, Galizi R, Kranjc N, Burt A, Beaghton AK, Nolan T, Crisanti A (2018) A CRISPR-Cas9 gene drive targeting doublesex causes complete population suppression in caged A. gambiae mosquitoes. […]

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Plant gene editing through de novo induction of meristems

In vitro cell culture is a bottleneck in the production of transgenic and genome-edited plants: in only a few species it is possible to develop new, modified plants from cell cultures. Furthermore, It is a lengthy process in which new and unintended mutations can occur. This study here describes a new method that can be […]

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